DnaPromix project
by Ivano Zara & Renato Schiavon
MIX PCR

Stock


Input Stock concentrations
Buffer x
MgCl2 mM
dNTPs mM
Primer F μM
Primer R μM
DNA polym.
Phusion 2U/μl
U/μl


Buffer: 10X
MgCl++: 50 mM
dNTPs: 10 mM
Primer: 10 uM
Taq: 5 U/ul
sample
Volumes (μl)

[concentration]
final
set
suggested
H2O
0.00
   
Buffer
x
1
MgCl2
mM
1.5
dNTPs
mM
0.2
Primer F
μM
0.35±0.25
Primer R
μM
0.35±0.25
DNA polymerase
U/μl
0.015 Taq
0.025 Pfu
0.02 Phu.
Template
 M
1e-21÷1e-15 M
 
Please insert sample total volume and template volume (if the case)
- Clicking upper arrow, single component volumes are accepted.
- Clicking lower arror, single component concentrations are accepted.
n° samples

Total mix

  Volumes μl
Vol.Tot.
0.00
H2O
0.00
Buffer
0.00
MgCl2
0.00
dNTPs
0.00
Primer F
0.00
Primer R
0.00
DNA pol.
0.000
Template
0.00
Concentration Template Conversion
Genomic size (bp)
concentration in weight
ng/μl
 
Concentration in molar
M
 
For printer
           Stock       Conc.Sample   Vol.Sample μl      μl  x  sample
Vol Tot 0.00 0.00
----------------------------------------------------------------------
H2O 0.00 0.00
Buffer 0.00 1.00 1X 0.00 0.00
MgCl2 0.00 mM 1.50 mM 0.00 0.00
dNTPs 0.00 μM 0.20 mM 0.00 0.00
Primer for 0.00 μM 0.35 μM 0.00 0.00
Primer REV 0.00 μM 0.35 μM 0.00 0.00
DNA polym. 0.00 U/μl 0.015 U/μl 0.000 0.000